Sperm viability

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Semen analysis is an important fertility test for infertile couples, and the test should be done before any treatments even "just clomid " are prescribed. Sometimes referred to as sperm count testing, a real semen analysis includes much more than just a sperm count. This is why every infertile couple must make sure the male partner is tested.

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In many species sperm competition selects for large ejaculates while females eliminate or disable a high percentage of the spermatozoa in their storage organs in order to control paternity. Therefore, sperm viability is an excellent measure of the reproductive success of both males and females. Here we assess the viability of spermatozoa in males, freshly mated queens and old queens at the end of a colony cycle of the monandrous bumblebee Bombus terrestris.

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There are six main criteria for healthy sperm:. Sperm also need to have the right number of chromosomes for a successful pregnancy. A breakdown in any of these criteria can result in male-factor infertility.

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By Sandhya R. Sperm forward motility is an essential parameter in mammalian fertilization. The purified sperm motility-initiating protein MIP from caprine epididymal plasma as well as the forward motility-stimulating factor FMSF and motility-stimulating protein MSP from buffalo and goat serum, respectively, have high efficacy to initiate or increase motility in nonmotile or less motile sperm.

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Sperm viability vitality should be assessed if a low percentage of sperm are progressively motile, eg. Since motile cells are inherently viable, a viability assessment may not be necessary when motility is high. This test is important to determine if the non-motile spermatozoa are alive or dead.

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The fundamental test for male infertility, semen analysis, is mostly a manually performed subjective and time-consuming process and the use of automated systems has been cost prohibitive. We have previously developed an inexpensive smartphone-based system for at-home male infertility screening through automatic and rapid measurement of sperm concentration and motility. Here, we assessed the feasibility of using a similar smartphone-based system for laboratory use in measuring: a Hyaluronan Binding Assay HBA score, a quantitative score describing the sperm maturity and fertilization potential in a semen sample, b sperm viability, which assesses sperm membrane integrity, and c sperm DNA fragmentation that assesses the degree of DNA damage.

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Sperm motility describes the ability of sperm to move properly through the female reproductive tract internal fertilization or through water external fertilization to reach the egg. Sperm motility can also be thought of as the qualitywhich is a factor in successful conception ; sperm that do not "swim" properly will not reach the egg in order to fertilize it. Sperm motility in mammals also facilitates the passage of the sperm through the cumulus oophorus a layer of cells and the zona pellucida a layer of extracellular matrixwhich surround the mammalian oocyte.

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In humans, sperm DNA fragmentation rates have been correlated with sperm viability rates. Reduced sperm viability is associated with high sperm DNA fragmentation, while conversely high sperm viability is associated with low rates of sperm DNA fragmentation. We postulated that in some men, the sperm viability assay could predict the sperm DNA fragmentation rates. This in turn could reduce the need for sperm DNA fragmentation assay testing, simplifying the infertility investigation and saving money for infertile couples.


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